ESI-Q-FT-ICR mass spectrometer

Name and Model: Bruker APEX-Qe FT-ICR mass spectrometer

Contact Person: Janne Jänis

Year of Manufacture: 1994

Year of Installation: 1994

General Technical Information: Ultra high resolution electrospray ionization APEX-Qe hybrid quadrupole Fourier transform ion cyclotron resonance mass spectrometer

Desrcription of Use:

• High mass accuracy compound elucidation
• Structural and functional characterization of biomolecules
• Structural and functional characterization of supramolecules
• Structural and functional characterization of metalclusters
• Native mass spectrometry
• Petroleum product analysis
• High end proteomics studies
• Protein/Protein or Protein/Ligand Interactions
• Gas phase chemistry (ion-molecule reactions, H/D-exchange, CID, ECD, IRMPD)

Key Features and Accessories:

• Bruker Apollo II Ion-Funnel ESI source
• Bruker On-Line Nanospray source
• 4.7 T magnet (12 T from December 2010)

• Gas phase ion-molecule reactions in ICR-cell (reaction rate determination)
• Gas phase H/D-exchange reactions (determination of labile hydrogens and H-bonding)
• Energy-resolved collision induced dissociation (CID)
• Electron capture dissociation (ECD)
• Infrared multiphoton dissociation (IRMPD)

Key Specifications:

• Mass Range: 100 – 10,000 m/z (transmission with RF only), 100 – 6,000 m/z (mass selective)
• Mass accuracy: < 1,0 ppm, m/z range 100 - 1500 (internal),< 1,5 ppm, m/z range 100 – 1500 (external)
• Resolving Power @ m/z 400: > 900,000 (FWHH)
• Sensitivity (Ubiquitin): S/N> 10:1 for <1fmol (consumed), High mass (BSA 0,1mg/ml): S/N>10:1
• Electrospray (standard): (1μl/min – 1 ml/min)
• On-Line Nanospray (standard): (100nl/min – 500 nl/min)
• Isolation efficiency (Qh-Interface): >60% (LHRH)
• MS/MS Efficiency (Qh-Interface): >50% (LHRH)
• Multistage MS (MS3 guaranteed): LHRH MS/MS (collision cell) -> MS/MS/MS (Infinity Cell™)
• Sensitivity ECD: S/N> 10:1 for 50fmol (consumed)

Photograph:

Location: University of Eastern Finland

City: Joensuu